Hepatitis C Virus (HCV), Quantitative, RNA PCR (Nongraphical) With Reflex to Genotyping

CPT: 87522
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Synonyms

  • HCV Real-time PCR With Reflex to Genotype
  • HCV RNA Quantitation With Reflex to Genotype

Special Instructions

This procedure does not provide serial monitoring; it is intended for one-time use only. If serial monitoring is required, please order test 550100.


Expected Turnaround Time

3 - 5 days



Specimen Requirements


Specimen

Serum or plasma


Volume

3 mL


Minimum Volume

1 mL (Note: This volume does not allow for repeat testing.)


Container

Gel-barrier tube, lavender-top (EDTA) tube, or plasma preparation tube (PPT™)


Collection

Centrifuge sample within 24 hours of collection. Transfer serum/plasma to a screw-cap polypropylene transport tube. Ship frozen (preferred). Plasma from a PPT™ can be frozen and shipped in situ. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit a separate frozen specimen for each test requested.


Storage Instructions

Freeze (preferred) or refrigerate.


Stability Requirements

Temperature

Period

Room temperature

24 hours (stability provided by manufacturer or literature reference)

Refrigerated

72 hours (stability provided by manufacturer or literature reference)

Frozen

6 weeks (stability provided by manufacturer or literature reference)


Causes for Rejection

Incorrect anticoagulant; PPT™ not centrifuged


Test Details


Use

This test is used for quantitation of hepatitis C virus with reflex to HCV genotype if viral load is >1000 IU/mL.


Limitations

The quantifiable range of the assay is 15 IU/mL to 100,000,000 IU/mL.


Methodology

Cobas® AmpliPrep/Cobas® TaqMan® HCV Test v 2.0. See Hepatitis C Virus (HCV) Genotyping, Nonreflex [550475] for more information.


References

Kleiber J, Walter T, Haberhausen G, Tsang S, Babiel R, Rosenstraus M. Performance characteristics of a quantitative, homogeneous TaqMan RT-PCR test for HCV RNA. J Mol Diagn. 2000 Aug; 2(3):158-166. 11229521

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
550090 HCV RNA by PCR, Qn Rfx Geno 11011-4 550028 Hepatitis C Quantitation IU/mL 11011-4
550090 HCV RNA by PCR, Qn Rfx Geno 11011-4 550051 HCV log10 log10 IU/mL 38180-6
550090 HCV RNA by PCR, Qn Rfx Geno 11011-4 550306 Test Information: 77202-0
550090 HCV RNA by PCR, Qn Rfx Geno 11011-4 551222 HCV Genotype 32286-7
Reflex Table for Hepatitis C Quantitation
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550040 HCV RNA (International Units) 550029 HCV RNA (International Units) IU/mL 11011-4
Reflex Table for Hepatitis C Quantitation
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550040 HCV RNA (International Units) 550032 HCV log10 log10 IU/mL 38180-6
Reflex Table for HCV Genotype
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550486 Hepatitis C Genotype 550513 Hepatitis C Genotype 32286-7
Reflex 2 550614 Hepatitis C Genotype 550614 Hepatitis C Genotype 32286-7

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