Assays

Molecular Assays

PCR assays:

• Viral Load Assays; HBV, HCV, HIV, CMV, RSV, HDV, HRV, FLU (Roche, Abbott, in-house LDT)
• HCV Genotyping (GenMark)
• Qualitative PCR detection assays
   

Reverse-hybridization assays:

• HCV and HBV genotyping (GenMark RVP)
   

Sequencing Assays:

Dye Terminator (Sanger)

• Bacterial identification
• HCV Genotyping
   

Next Generation:

• HIV/SIV env (Ab)
• HIV gag (CTL)
• FLU NA, HA
• RSV F, G, SH
• HBV WGS (C,P,S,X)
• HDV WGS, ΔAg
   

Specialty assays:

• HIV coreceptor tropism
• Clonal analyses
• Epitope mapping
• Virus panels

Additional specialty assays can be seamlessly accessed through LabCorp including Monogram Biosciences, a member of LabCorp’s Specialty Testing Group.

Immunoassays

Immunochemistry and biomarker assays supported in facilities
including BSL3 environment:

• Enzyme-Immuno-Assays (EIA)
• Radioimmunoassays (RIA)
• Antibody titers in various matrices
• Antigen-Antibody binding assays employing Biacore and Octet platforms
• Measurement of cytokine levels and other biomarkers in various matrices using a variety of immunoassay platforms including ELISA, Meso Scale Discovery (MSD), Luminex, Gyros and Singulex

Functional and Cell-Based Assays

Flow Cytometry:      

• Immunophenotyping
• Immune cell activation and proliferation markers
• Immune cell mediated killing (including T-cell and NK-cell mediated killing)
• Intracellular cytokine staining
• Viral Ab binding
   

Cell based assays:           

• T-cell proliferation assay
• Cytokine release assay
• Enzyme Linked ImmunoSpot (ELISPOT) assay
• Assays to determine antibody associated functions such as Complement Dependent Cytotoxicity (CDC), Antibody Dependent Cellular Cytotoxicity (ADCC)
• Neutralizing Ab Assays
  • HIV/SIV
  • FLU
  • RSV
  • Ebola
     

Bactericidal assays:

• OPA - Opsonophagocytic activity assay 
• SBA -  Serum Bactericidal Activity assay