Norovirus Detection, Real-time PCR

CPT: 87798(x2)
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Synonyms

  • Human Calicivirus
  • Norwalk Virus

Expected Turnaround Time

4 - 5 days


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Specimen Requirements


Specimen

Stool


Volume

1 mL


Minimum Volume

0.2 mL (Note: This volume does not allow for repeat testing.)


Container

Sterile container or Para-Pak® Orange


Storage Instructions

Refrigerate. Samples in Cary-Blair medium (Para-Pak® Orange) can be kept at room temperature.


Stability Requirements

TemperaturePeriod
Room temperature

Sterile container: 7 days;

Cary-Blair medium: 5 days

Refrigerated

Sterile container: 7 days;

Cary-Blair medium: 14 days

Frozen

Sterile container: 7 days;

Cary-Blair medium: Unstable


Causes for Rejection

Quantity not sufficient for analysis; specimen too old; leaking or broken tube; frozen sample in Cary-Blair medium (Para-Pak® Orange)


Test Details


Use

This test is intended to be used as an aid in the diagnosis of infections caused by noroviruses, and to differentiate viruses belonging to Norovirus genogroup 1 (eg, Norwalk virus) and from those belonging to Norovirus genogroup 2 (e.g., Snow Mountain agent).


Limitations

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.


Methodology

Real-time reverse transcriptase polymerase chain reaction (RT-PCR)


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