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Hybrid (LBA)-LC-MS/MS methodology covers the different dimensions of ADC bioanalysis

November 8, 2024
China Bioanalysis Forum 2024 -- An antibody-drug conjugate (ADC) is generally composed of a small-molecule drug, also known as a payload, and an antibody or antibody fragment, conjugated together by a chemical linker. In accordance with recommended guidelines, it is advised to primarily detect the conjugated antibody/Ab-conjugated payload (ADC), total antibody (conjugated antibody and unconjugated antibody), and free small molecule compounds in pharmacokinetic (PK) studies of ADCs. So, three bioanalytical methods are typically employed in ADC bioanalysis: free toxin PK assay, ADC PK assay (Ab-conjugated payload), total antibody PK assay. Recently the hybrid (LBA)-LC-MS/MS has been used on ADC bioanalysis and has covered ADC PK assay and total antibody PK assay, plus regular LC-MS/MS method for free toxin assay, LC-MS/MS platform provides additional valuable solutions for ADC bioanalysis.