DMDG Open 2024 -- Understanding the metabolic clearance and metabolism of xenobiotics across species is important for ensuring appropriate toxicological data is obtained, and for the successful prediction of human pharmacokinetics. Obtaining reliable in vitro metabolic clearance data and assessing the metabolite profiles of metabolically stable compounds using traditional hepatocytes suspensions has been challenging due to limited enzyme stability. Several test systems now offer extended enzyme stability and incubation times to more accurately assess the metabolic stability of metabolic stable compounds. Here the HμREL® co‑cultured hepatic model, which offers primary cryopreserved hepatocytes cultured together with cells of a non-parenchymal, stromal type and the HEPATOPAC® co-cultured hepatic model, which form hepatocyte islands surrounded by supportive stromal cells, were assessed and compared.

• Download