ASR 2024 -- Adipose tissue biopsy is a useful technique for obtaining adipose samples for analysis in numerous research applications such as metabolic and cardiovascular disease studies. Due to the abundance of adipose and ease of access, the subcutaneous (SQ) space is the most common location to perform adipose biopsies. However, obtaining adequate samples can be a challenge in animals with minimal SQ adipose tissue, such as the commonly used cynomolgus macaque (Macaca fascicularis). Based on observations during macaque laparotomy and laparoscopy procedures, we hypothesized that the falciform ligament and omentum may be a useful and convenient source of adipose tissue in cynomolgus macaques. This led us to develop a minimally invasive alternative approach to adipose tissue acquisition in cynomolgus macaques lacking sufficient SQ fat for standard SQ collection. By applying standard in-house laparoscopic tissue biopsy procedures, we were able to successfully obtain repeat adipose tissue samples from cynomolgus macaques. This technique provides a convenient solution to the lack of SQ adipose tissue in young or underconditioned cynomolgus macaques. Additionally, this represents a refinement to current adipose tissue collection techniques in young or underconditioned cynomolgus macaques in which more and larger incisions, as compared to laparoscopic approach, are often required to obtain adequate sampling. 

• Download