Creatine Kinase (CK), Total

Serum (preferred) or plasma

1 mL

0.7 mL (Note: This volume does not allow for repeat testing.)

Red-top tube, gel-barrier tube, or green-top (lithium heparin) tube. Do not use oxalate, EDTA, or citrate plasma.

Avoid exercise before venipuncture. Increases may be anticipated in the immediate postoperative period following surgical procedures involving incision through muscle.

Test for acute myocardial infarct and for skeletal muscular damage; elevated in some patients with myxedema (hypothyroidism), malignant hyperthermia syndrome, and muscular dystrophy. CK is a marker for Duchenne muscular dystrophy, with elevations of 20 to 200 times normal.¹ CK is increased in female carriers of this X-linked disease, and in muscular stress, in polymyositis, dermatomyositis, and with muscle trauma. Elevated in myocarditis. Documentation of postictal state (recent grand mal seizure). Extremely high values are seen in some instances of myositis and in the postictal state. CK may be elevated in a number of entities, including the eosinophilia-myalgia syndrome.² Marked increases occur with rhabdomyolysis including that with cocaine intoxication.³ CK is sometimes increased with cerebrovascular accident. Malignancy (advanced) may show increased CK.⁴ Cardioversion with multiple shocks may release CK-MB and may result in a false-positive diagnosis of myocardial infarction.⁵ Low CK may reflect decreased muscle mass. It has been reported with a number of entities, including metastatic neoplasia, patients with steroid therapy, with alcoholic liver disease⁶ and with connective tissue diseases.⁷ Overnight bedrest may lower CK 10% to 20%.

Intramuscular injections increase serum CK activity. Elevated following exercise. Normal at onset of acute MI unless the subject has been exercising or doing physical work. Elevation of CK following acute MI may not be observed until six or more hours after onset. CK returns to normal in approximately 48 to 72 hours after acute MI. Total CK can be normal in acute MI, when CK-MB is increased. Low CK does not rule out myositis in patients with the connective tissue diseases.⁷ Decreased with pregnancy.

Kinetic − 340 nm spectrophotometric

See table.

High CK is found after trauma, surgery, and exercise; these entities may not be accompanied by elevation of CK-MB. To distinguish myoglobinuria from hemoglobinuria, serum CK and LD may be helpful. CK is normal with uncomplicated hemolysis but LD and LD₁ usually are increased. When myoglobin is released, 40-fold elevation of CK may be anticipated with only moderate increase in serum LD and increased LD₅.⁸