Chromosome Five-cell Count Plus Microarray (Reveal®), Amniotic Fluid

Amniotic fluid (for direct array and abbreviated amnio chromosome analysis)

Gender by ultrasound is required. Please submit maternal blood (sodium heparin or EDTA) for maternal cell contamination (MCC) studies.

If the specimen does not meet minimum DNA quality and quantity requirements, array testing will be performed on cultured material. If cultures are needed, additional days will be required to complete testing. A notification will be sent to the client if cultures are necessary.

25 mL amniotic fluid for concurrent array and chromosome analysis

12 mL amniotic fluid (minimum volume may delay results; cells would need to be cultured for microarray analysis)

Sterile plastic conical tube

The test allows prenatal detection of chromosomal aneuploidy, and is used to rule out tetraploidy and rearrangements not detected by array, such as balanced translocations and inversions. It also helps to clarify array abnormalities to determine if a structural rearrangement, marker, or isochromosome is present. Array can only provide copy number imbalances and cannot determine structure of an abnormality. The SNP assay will detect chromosomal imbalances that could be associated with developmental delay/congenital anomalies. It provides detection of possible uniparental disomy of any chromosome, the percent and location of homozygosity, including the degree of identity by descent.

This SNP assay does not detect balanced rearrangements, low-level mosaicism (<10%), marker chromosomes that only contain heterochromatin or tetraploidy.

This test was developed and its performance characteristics determined by LabCorp. It has not been cleared or approved by the Food and Drug Administration.

Five-cell G-Band Chromosome analysis. SNP microarray analysis is performed using the Cytoscan® HD platform, which uses more than 743,000 SNP probes and 1,953,000 NPCN probes with a median spacing of 0.88 kb.